Production of sugars from mixed hardwoods for use in the synthesis of sugar fatty acid esters catalyzed by immobilized‐stabilized derivatives of <i>Candida antarctica</i> lipase B

The synthesis of sugar fatty acid esters (SFAEs) from lignocellulosic biomass and oleic (C18:1) was catalyzed by immobilized-stabilized derivatives Candida antarctica lipase B in a methyl ethyl ketone medium. After steam-explosion pretreatment mixed hardwoods enzymatic hydrolysis at 15%wt solids, xylose glucose were purified/concentrated to mass ratio ~3 1. These sugars superior commercial as the carbohydrate source for esterification reaction terms conversions. highest conversions obtained using 1.5% w/v Novozyme 435 (N435, uncoated) biocatalyst SFAEs. Coating N435 with polyethyleneimine (PEI) prevented enzyme leakage into medium produced 35% 50% higher SFAEs, respectively, same loading. six 24 h reuse cycles PEI-coated N435, conversion decreased 44%, while 65% reduction observed uncoated lipase. Mass spectrometry analysis confirmed production mono- di-esters. Our purified product presented an emulsion capacity (EC) close that ester ECs oleate, laurate, palmitate synthesized previous studies.